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KMID : 0364820150510010075
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Korean Journal of Microbiology
2015 Volume.51 No. 1 p.75 ~ p.80
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Characterization and optimum production condition of extracellular protease from Pseudoalteromonas donghaensis HJ51
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Oh Ji-Sung
Choi Yoon-Soo
Roh Dong-Hyun
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Abstract
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Pseudoalteromonas donghaensis HJ51, isolated from the East Sea, has been reported as a novel strain to produce extracellular protease. Crude supernatant was used to determine optimal activity and optimal production conditions for the enzyme. It was found that the optimal temperature and pH of the protease were 40캜 and pH 7.5?0.5, respectively. The enzyme activity was kept to 88% at the pH 11. In metal requirement analysis, the enzyme exhibited the highest activity when 10 mM Fe 3+ was supplied. While supplementation of additional carbon sources used in study showed no positive effect on cell growth and enzyme activity, the addition of beef extract, tryptone, or casamino acids instead of peptone of PY-ASW containing 1% glucose increased enzyme production to 21, 7, 4%, respectively. Taken together these properties, the enzyme produced from P. donghaensis HJ51 can be applied to the industries that require protease activity under alkaline pH and low temperature.
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KEYWORD
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Pseudoalteromonas donghaensis HJ51, optimal condition, protease production
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